Publications: Research Articles


Parker J.L, Kato T, Kuteyi G, Sitsel O, Newstead S. (2023) Molecular basis for selective uptake and elimination of organic anions in the kidney by OAT1. Nature Structural & Molecular Biology.

Loebel M, Salphati, S.P, El Omari K, Wagner A, Tucker S.J, Parker J.L, Newstead S. (2022) Structural basis for proton coupled cystine transport by cystinosin. Nature Communications.

Fiore A, Zeitler L, Russier M, Groß A, Hiller MK, Parker JL, Stier L, Köcher T, Newstead S, Murray PJ. (2022) Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling. Mol Cell.

Parker J.L, Deme J.C, Zhiyi Wu, Kuteyi G, Jiandong Huo, Raymond J. Owens, Biggin P.C, Lea S.M, Newstead S. (2021) Cryo-EM structure of PepT2 reveals structural basis for proton-coupled peptide and prodrug transport in mammals. SciAdv

Parker J.L, Deme J.C, Kolokouris D, Kuteyi G, Biggin P.C, Lea S.M, Newstead S. (2021) Molecular basis for redox control by the human cystine/glutamate antiporter System xc. Nature Communications

Adams O, Deme J.C, Parker J.L, the CRyPTIC Consortium, Fowler P.W., Lea S.M, Newstead S. (2021) Cryo-EM structure and resistance landscape of M. tuberculosis MmpL3: An emergent therapeutic target. Structure.

Parker J.L, Deme J.C, Kuteyi G, Wu Z, Huo J, David Goldman I, Owens R.J, Biggin P.C, Lea S.M, Newstead S. (2021) Structural basis of antifolate recognition and transport by PCFT. Nature.

Gerondopoulos A, Bräuer P, Sobajima T, Wu Z, Parker JL, Biggin PC, Barr FA, Newstead S. (2021) A signal capture and proofreading mechanism for the KDEL-receptor explains selectivity and dynamic range in ER retrieval. Elife.

Wu Z, Newstead S, Biggin PC. (2020) The KDEL trafficking receptor exploits pH to tune the strength of an unusal short hydrogen bond. Sci Rep.

van Dijk FS, et al. (2020) Interaction between KDELR2 and HSP47 as a Key Determinant in Osteogenesis Imperfecta Caused by Bi-allelic Variants in KDELR2. Am J Hum Genet.

Zimmermann I, Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsama ER, Seeger MA. (2020) Generation of synthetic nanobodies against delicate proteins. Nat Protoc.

Parker JL, Corey R, Stansfeld P, Newstead S. (2019) Structural basis for substrate specificity and regulation of nucleotide sugar transporters in the lipid bilayer. Nature Communications.

Zhiyi W, Alibay I, Newstead S, Biggin PC. (2019) Proton control transitions in an amino acid transporters. Biophysical Journal.

Bräuer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr FA, Newstead S. (2019) Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor. Science.

Minhas GS, Newstead S. (2019) Structural basis for prodrug recognition by the SLC15 family of proton coupled peptide transporters. PNAS. 

Minhas GS, Bawdon D, Herman R, Rudden M, Stone AP, James AG, Thomas GH, Newstead S. (2018) Structural basis of malodour precursor transport in the human axilla. eLIFE 

Minhas GS, Newstead S. (2018) Structural basis for prodrug recognition by the SLC15 family of proton coupled peptide transporters. BioRxiv DOI

McIlwain BC, Newstead S, Stockbridge RB. (2018) Cork-in-Bottle Occlusion of Fluoride Ion Channels by Crystallization Chaperones. Structure

Jungnickel, EJK, Parker JL, Newstead S. (2018) Structural basis for amino acid transport by the CAT family of SLC7 transporters. Nature Communications

Parker JL, Newstead S. (2017) Structural basis of nucleotide sugar transport across the Golgi membrane. Nature 

Parker JL, Li C, Brinth A, Wang Z, Vogeley L, Solcan N, Ledderboge-Vucinic G, Swanson JMJ, Caffrey M, Voth GA, Newstead S. (2017) Proton movement and coupling in the POT family of peptide transporters. PNAS

Byrne EF, Sircar R, Miller PS, Hedger G, Luchetti G, Nachtergaele S, Tully MD, Mydock-McGrane L, Covey DF, Rambo RP, Sansom MS, Newstead S, Rohatgi R, Siebold, C. (2016) Structural basis of Smoothened regulation by its extracellular domains. Nature

Zhang F, Hanson SM, Jara-Oseguera A, Krepkiy D, Bae C, Pearce LV, Blumberg PM, Newstead S, Swartz KJ. (2016) Engineering vanilloid-sensitivity into the rat TRPV2 channel. eLife

Samsudin F, Parker JL, Sansom MS, Newstead S, Fowler PW. (2016) Accurate prediction of ligand affinities for a peptide transporter. Cell Chemistry & Biology.

Stockbridge RB, Kolmakova-Partensky L, Shane T, Koide A, Koide S, Miller C, Newstead S.  (2015) Crystal structures of a double-barrelled fluoride ion channel. Nature

Beale JH, Parker JL, Samsudin F, Barreyy AL, Senan A, Bird LE, Scott D, Owens RJ, Sansom MS, Tucker SJ, Meredith D, Fowler PW, Newstead S. (2015) Crystal structure of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport. Structure 23, 1889-99.

Stansfeld PJ, Goose JE, Caffrey M, Carpenter EP, Parker JL, Newstead S, Sansom MS, et al., (2015) MemProtMD: Automated Insertion of Membrane Protein Structures into Explicit Lipid Membranes. Structure

Hanson SM, Newstead S, Swartz KJ, Sansom MS. (2015) Capsaicin interaction with TRPV1 channels in a lipid bilayer: molecular dynamics simulation. Biophys J 

Fowler PW, Orwick-Rydmark M, Radestock S, Solan N, Dijkman PM, Lyons JA, Kowk J, Caffrey M, Watts A, Forrest LR, Newstead S. (2015) Gating topology of the proton coupled oligopeptide symporters. Structure.

Parker JL, Mindell JA, Newstead S. (2014). Thermodynamic evidence for a dual transport mechanism in a POT peptide transporter. eLife. 

Parker JL, Newstead S. (2014). Method to increase the yield of eukaryotic membrane protein expression in Saccharomyces cerevisiae for structural and functional studies. Protein Sci.

Lyons JA, Parker JL, Solcan N, Brinth A, Li D, Shah ST, Caffrey M, Newstead S. (2014). Structural basis for polyspecificity in the POT family of proton-coupled oligopeptide transporters. EMBO Rep.

Parker JL & Newstead S. (2014). Molecular basis for nitrate uptake by the plant nitrate transporter NRT1.1. Nature.

Lee C, Kang HJ, von Ballmoos C, Newstead S, Uzdavinys P, Dotson DL, Iwata S, Beckstein O, Cameron, AD, Drew D. (2013). A two-domain elevator mechanism for sodium/proton antiport. Nature.

Doki S, Kata HE, Solcan N, Iwaki M, Koyama M, Hattori M, Iwase N, Tsukazaki T, Sugita Y, Kandori H, Newstead S, Ishitani R, Nureki O. (2013). Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT. PNAS.

Parker, J., Newstead, S., (2013). Phasing statistics for alpha helical membrane protein structuresProtein Sci.

Parker, J., Newstead, S., (2012). Current trends in alpha helical membrane protein crystallization; an update.  Protein Sci.

Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S. (2012). Alternating access mechanism in the POT family of oligopeptide transporters. EMBO J.

Bill, R.M., Henderson, P.J., Iwata, S., Kunji, E.R., Neutze, R., Newstead, S., Poolman, B., Tate, C.G., Vogel, H. (2011). Overcoming the barriers to membrane protein structure determination. Nat Biotechnol. 

Newstead, S., Drew, D., Cameron, A.D., Postis, V.L.G., Xia, X., Fowler, P.W., Ingram, J.C., Carpenter, E.P., Sansom, M.S.P., McPherson, M., Baldwin, S.A., Iwata, S. (2011). Crystal structure of a prokaryotic homologue of the mammalian oligopeptide proton symporters, PepT1 and PepT2. EMBO J.

Sonoda Y, Newstead S, Hu NJ, Alguel Y, Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron AD, Byrne B, Iwata S, Drew D. (2011). Benchmarking membrane protein detergent stability for improving throughput of high resolution X-ray structures. Structure

Sonada, Y., Cameron, A., Newstead, S., Omote, H., Moriyama, Y., Kasahara, M., Iwata, S., Drew, D. (2010). Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. FEBS Lett.

Newstead, S., Fowler, P.W., Bilton, P., Carpenter, E.P., Sadler, P., Campopiano, D., Sansom, M.S.P., and Iwata, S. (2009). Insights into how nucleotide-binding domains power ABC transport. Structure

Newstead, S., Hobbs, J., Jordan, D., Carpenter, E.P., and Iwata, S. (2008). Insights into outer membrane protein crystallisation. Molecular Membrane Biology

Drew, D., Klepsch, M.M., Newstead, S., Flaig, R., De Gier, J.-W., Iwata, S., and Beis, K. (2008). The structure of the efflux pump AcrB in complex with bile acid. Molecular Membrane Biology

Newstead, S., Ferrandon, S., and Iwata, S. (2008). Rationalizing alpha-helical membrane protein crystallization. Protein Sci 

Drew, D., Newstead, S., Sonoda, Y., Kim, H., von Heijne, G., and Iwata, S. (2008). GFP- based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat Protocols .

Newstead, S., Kim, H., von Heijne, G., Iwata, S., and Drew, D. (2007). High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. PNAS.

Gutmann, D.A., Mizohata, E., Newstead, S., Ferrandon, S., Postis, V., Xia, X., Henderson, P.J., van Veen, H.W., and Byrne, B. (2007). A high-throughput method for membrane protein solubility screening: the ultracentrifugation dispersity sedimentation assay. Protein Sci 16, 1422-1428.