Publications: Research Articles

2007-2018

Minhas GS, Newstead S. (2018) Structural basis for prodrug recognition by the SLC15 family of proton coupled peptide transporters. BioRxiv DOI https://doi.org/10.1101/454116.

Minhas GS, Bawdon D, Herman R, Rudden M, Stone AP, James AG, Thomas GH, Newstead S. (2018) Structural basis of malodour precursor transport in the human axilla. eLIFE DOI: doi: 10.7554/eLife.34995.

McIlwain BC, Newstead S, Stockbridge RB. (2018) Cork-in-Bottle Occlusion of Fluoride Ion Channels by Crystallization Chaperones. Structure DOI: 10.1016/j.str.2018.02.004.

Jungnickel, EJK, Parker JL, Newstead S. (2018) Structural basis for amino acid transport by the CAT family of SLC7 transporters. Nature Communications DOI: 10.1038/s41467-018-03066-6

Parker JL, Newstead S. (2017) Structural basis of nucleotide sugar transport across the Golgi membrane. Nature, 551, 521-24.

Parker JL, Li C, Brinth A, Wang Z, Vogeley L, Solcan N, Ledderboge-Vucinic G, Swanson JMJ, Caffrey M, Voth GA, Newstead S. (2017) Proton movement and coupling in the POT family of peptide transporters. Proc Natl Acad Sci U S A. 114, 3182-13187.

Byrne EF, Sircar R, Miller PS, Hedger G, Luchetti G, Nachtergaele S, Tully MD, Mydock-McGrane L, Covey DF, Rambo RP, Sansom MS, Newstead S, Rohatgi R, Siebold, C. (2016) Structural basis of Smoothened regulation by its extracellular domains. Nature, 535, 517-22.

Zhang F, Hanson SM, Jara-Oseguera A, Krepkiy D, Bae C, Pearce LV, Blumberg PM, Newstead S, Swartz KJ. (2016) Engineering vanilloid-sensitivity into the rat TRPV2 channel. Elife May 13:5.

Samsudin F, Parker JL, Sansom MS, Newstead S, Fowler PW. (2016) Accurate prediction of ligand affinities for a peptide transporter. Cell Chemistry & Biology. 23, 299-309.

Stockbridge RB, Kolmakova-Partensky L, Shane T, Koide A, Koide S, Miller C, Newstead S.  (2015) Crystal structures of a double-barrelled fluoride ion channel. Nature 525, 548-51.

Beale JH, Parker JL, Samsudin F, Barreyy AL, Senan A, Bird LE, Scott D, Owens RJ, Sansom MS, Tucker SJ, Meredith D, Fowler PW, Newstead S. (2015) Crystal structure of the Extracellular Domain from PepT1 and PepT2 Provide Novel Insights into Mammalian Peptide Transport. Structure 23, 1889-99.

Stansfeld PJ, Goose JE, Caffrey M, Carpenter EP, Parker JL, Newstead S, Sansom MS, et al., (2015) MemProtMD: Automated Insertion of Membrane Protein Structures into Explicit Lipid Membranes. Structure 23, 1350-61.

Hanson SM, Newstead S, Swartz KJ, Sansom MS. (2015) Capsaicin interaction with TRPV1 channels in a lipid bilayer: molecular dynamics simulation. Biophys J. 108, 1425-34.

Fowler PW, Orwick-Rydmark M, Radestock S, Solan N, Dijkman PM, Lyons JA, Kowk J, Caffrey M, Watts A, Forrest LR, Newstead S. (2015) Gating topology of the proton coupled oligopeptide symporters. Structure. 23, 290-301.

Parker JL, Mindell JA, Newstead S. (2014). Thermodynamic evidence for a dual transport mechanism in a POT peptide transporter. Elife. 2014 Dec 2;3.

Parker JL, Newstead S. (2014). Method to increase the yield of eukaryotic membrane protein expression in Saccharomyces cerevisiae for structural and functional studies. Protein Sci. 9, 1309-14.

Lyons JA, Parker JL, Solcan N, Brinth A, Li D, Shah ST, Caffrey M, Newstead S. (2014). Structural basis for polyspecificity in the POT family of proton-coupled oligopeptide transporters. EMBO Rep. 8, 886-93.

Newstead, S. (2014). Molecular insights into proton coupled peptide transport in the PTR family of oligopeptide transporters.  Biochim Biophys Acta. S0304-4165.

Parker JL & Newstead S. (2014). Molecular basis for nitrate uptake by the plant nitrate transporter NRT1.1. Nature. 507, 68-72.

Lee C, Kang HJ, von Ballmoos C, Newstead S, Uzdavinys P, Dotson DL, Iwata S, Beckstein O, Cameron, AD, Drew D. (2013). A two-domain elevator mechanism for sodium/proton antiport. Nature. 501, 573-577.

Doki S, Kata HE, Solcan N, Iwaki M, Koyama M, Hattori M, Iwase N, Tsukazaki T, Sugita Y, Kandori H, Newstead S, Ishitani R, Nureki O. (2013). Structural basis for dynamic mechanism of proton-coupled symport by the peptide transporter POT. Proc Natl Acad Sci U S A 110, 11343-11348.

Parker, J., Newstead, S., (2013). Phasing statistics for alpha helical membrane protein structures.  Protein Sci. 22, 1664-1668.

Parker, J., Newstead, S., (2012). Current trends in alpha helical membrane protein crystallization; an update.  Protein Sci. 21, 1358-1365.

Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D, Iwata S, Newstead S. (2012). Alternating access mechanism in the POT family of oligopeptide transporters. EMBO J. 31, 3411 – 3421.

Bill, R.M., Henderson, P.J., Iwata, S., Kunji, E.R., Neutze, R., Newstead, S., Poolman, B., Tate, C.G., Vogel, H. (2011). Overcoming the barriers to membrane protein structure determination. Nat Biotechnol. 29, 335-340.

Newstead, S., Drew, D., Cameron, A.D., Postis, V.L.G., Xia, X., Fowler, P.W., Ingram, J.C., Carpenter, E.P., Sansom, M.S.P., McPherson, M., Baldwin, S.A., Iwata, S. (2011). Crystal structure of a prokaryotic homologue of the mammalian oligopeptide proton symporters, PepT1 and PepT2. EMBO J. 30, 417-426.

Sonoda Y, Newstead S, Hu NJ, Alguel Y, Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron AD, Byrne B, Iwata S, Drew D. (2011). Benchmarking membrane protein detergent stability for improving throughput of high resolution X-ray structures. Structure 19,17-25.

Sonada, Y., Cameron, A., Newstead, S., Omote, H., Moriyama, Y., Kasahara, M., Iwata, S., Drew, D. (2010). Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins. FEBS Lett. 584, 2539-2547.

Newstead, S., Fowler, P.W., Bilton, P., Carpenter, E.P., Sadler, P., Campopiano, D., Sansom, M.S.P., and Iwata, S. (2009). Insights into how nucleotide-binding domains power ABC transport. Structure 17, 1213-1222.

Newstead, S., Hobbs, J., Jordan, D., Carpenter, E.P., and Iwata, S. (2008). Insights into outer membrane protein crystallisation. Molecular Membrane Biology 8, 631-638.

Drew, D., Klepsch, M.M., Newstead, S., Flaig, R., De Gier, J.-W., Iwata, S., and Beis, K. (2008). The structure of the efflux pump AcrB in complex with bile acid. Molecular Membrane Biology 8, 677-682.

Newstead, S., Ferrandon, S., and Iwata, S. (2008). Rationalizing alpha-helical membrane protein crystallization. Protein Sci 17, 466-472.

Drew, D., Newstead, S., Sonoda, Y., Kim, H., von Heijne, G., and Iwata, S. (2008). GFP- based optimization scheme for the overexpression and purification of eukaryotic membrane proteins in Saccharomyces cerevisiae. Nat Protoc 3, 784-798.

Newstead, S., Kim, H., von Heijne, G., Iwata, S., and Drew, D. (2007). High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 104, 13936-13941.

Gutmann, D.A., Mizohata, E., Newstead, S., Ferrandon, S., Postis, V., Xia, X., Henderson, P.J., van Veen, H.W., and Byrne, B. (2007). A high-throughput method for membrane protein solubility screening: the ultracentrifugation dispersity sedimentation assay. Protein Sci 16, 1422-1428.